| Threshold | Creates a binary from a 3D color input. Tried to get the Cy5 channel in this example.  |
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| Simple Threshold | Another 3D thresholding option that only includes a slider (no cleaning). Used the same 5500-65535 bounds as 3D Threshold and more noise was picked up because Clean was not used. |
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| Bright Spots | A thresholding method that looks for bright spots on a dark background. Can adjust several parameters to refine the filter and choose the binary output to be areas of the threshold diameter or spots of a specified width.  |
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| Dark Spots | A thresholding method for finding dark spots against a bright background. Did not work very well on the example image since it is not brightfield.  |
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