When imaging, be sure to save your data to the local data drive (usually D, E, F, G, or H) on every microscope PC. NEVER save to C-drive. ALWAYS back up your data to a location you fully control. When done, you can upload your data to your cloud service, email it to yourself, or…

We can now scan slides in an automated fashion on most of the scanning confocals, spinning disk (SD and CrestV2), or wide field fluorescence (HCA). We can have the system detect tissue slices automatically using 4x and then collect high resolution tiles/z-stacks/etc. for focusing and stitching. It can then text you when done with the…

Much like a glass left out in the pouring rain, the glass will eventually overflow. Like that glass, digital detectors (PMTs on the scanning confocals, cameras on the other microscopes) can only hold so much signal; 0-4095 for 12-bit and 0-65535 for 16-bit. If the detector is full and you “pour” in more photons like…

With the addition of a new module inside of NIS-Elements on the A1SP, you can now make use of our awesome 4 laser, dual single-photon detector FLIM system right inside of NIS-Elements. Thus, doing time lapses, z-stacks, or other multiplexed FLIM experiments is as simple as running a green/red experiment now. You can also do…

One perpetual question that we get is, “How can I get my data?” Here is the answer and what I consider Best Practice. 1) SAVE your data during acquisition to a local data drive (usually D or E, NEVER C as it may be deleted automatically) 2) At session end, COPY that data to a…

Did you know that it is quite easy to make your time on LMF microscopes much more efficient? On most of our microscopes, you will find “ND acquisition” which is a module that allows you to combine various dimensions such as multicolor, time-lapse, z-stacks, and numerous x-y-z positions. Thus, instead of running a time-lapse on…