TECH HIGHLIGHT: MULTIDIMENSIONAL IMAGING

Did you know that it is quite easy to make your time on LMF microscopes much more efficient? On most of our microscopes, you will find “ND acquisition” which is a module that allows you to combine various dimensions such as multicolor, time-lapse, z-stacks, and numerous x-y-z positions. Thus, instead of running a time-lapse on one field of view (FOV) overnight, you could run it on 96 wells with 10 FOVs each thus resulting in 960 time-lapse datasets for the same amount of microscope time. Using the Perfect Focus System (PFS) is key to multipoint imaging. To use it, simply manually focus, note your z-value, then turn on PFS, and adjust offset knob to return to your z-value.